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R&D Systems recombinant mouse gdf7 protein

<t>GDF7</t> alleviates LPS-induced ALI and pulmonary dysfunction in mice. (a) Mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI, and serum GDF7 levels were detected using an ELISA kit after 12 h ( n = 6). (b) The levels of Gdf7 mRNA in lung tissues with or without LPS instillation were detected using quantitative real-time PCR ( n = 6). (c) The levels of GDF7 protein in lung tissues with or without LPS instillation were detected using western blot ( n = 6). (d-e) Mice were subcutaneously injected with <t>rmGDF7</t> (25 μ g per mouse), and serum and lung GDF7 levels in mice were detected using an ELISA kit after 24 h ( n = 6). (f) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI. After 12 h, fresh lungs were harvested for the analysis of LDH activity using a commercial kit ( n = 6). (g) Lung wet to dry ratio ( n = 6). (h) Total proteins in BALF ( n = 6). (i) Arterial blood gas analysis results ( n = 6). (j) Respiratory function was detected by Buxco, including pulmonary ventilation, lung compliance, and airway resistance ( n = 6). (k) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with a lethal dose of LPS (25 mg/kg). The survival rate was monitored every 12 h post-LPS treatment ( n = 20). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant.

Recombinant Mouse Gdf7 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+gdf7+protein/Recombinant+Mouse+GDF-7+Protein/pmc09800101-29-0-23
Average 92 stars, based on 7 article reviews

recombinant mouse gdf7 protein - by Bioz Stars, 2026-09

92/100 stars

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1) Product Images from "Growth Differentiation Factor 7 Prevents Sepsis-Induced Acute Lung Injury in Mice"

Article Title: Growth Differentiation Factor 7 Prevents Sepsis-Induced Acute Lung Injury in Mice

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

doi: 10.1155/2022/3676444

GDF7 alleviates LPS-induced ALI and pulmonary dysfunction in mice. (a) Mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI, and serum GDF7 levels were detected using an ELISA kit after 12 h ( n = 6). (b) The levels of Gdf7 mRNA in lung tissues with or without LPS instillation were detected using quantitative real-time PCR ( n = 6). (c) The levels of GDF7 protein in lung tissues with or without LPS instillation were detected using western blot ( n = 6). (d-e) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and serum and lung GDF7 levels in mice were detected using an ELISA kit after 24 h ( n = 6). (f) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI. After 12 h, fresh lungs were harvested for the analysis of LDH activity using a commercial kit ( n = 6). (g) Lung wet to dry ratio ( n = 6). (h) Total proteins in BALF ( n = 6). (i) Arterial blood gas analysis results ( n = 6). (j) Respiratory function was detected by Buxco, including pulmonary ventilation, lung compliance, and airway resistance ( n = 6). (k) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with a lethal dose of LPS (25 mg/kg). The survival rate was monitored every 12 h post-LPS treatment ( n = 20). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant.

Figure Legend Snippet: GDF7 alleviates LPS-induced ALI and pulmonary dysfunction in mice. (a) Mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI, and serum GDF7 levels were detected using an ELISA kit after 12 h ( n = 6). (b) The levels of Gdf7 mRNA in lung tissues with or without LPS instillation were detected using quantitative real-time PCR ( n = 6). (c) The levels of GDF7 protein in lung tissues with or without LPS instillation were detected using western blot ( n = 6). (d-e) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and serum and lung GDF7 levels in mice were detected using an ELISA kit after 24 h ( n = 6). (f) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI. After 12 h, fresh lungs were harvested for the analysis of LDH activity using a commercial kit ( n = 6). (g) Lung wet to dry ratio ( n = 6). (h) Total proteins in BALF ( n = 6). (i) Arterial blood gas analysis results ( n = 6). (j) Respiratory function was detected by Buxco, including pulmonary ventilation, lung compliance, and airway resistance ( n = 6). (k) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with a lethal dose of LPS (25 mg/kg). The survival rate was monitored every 12 h post-LPS treatment ( n = 20). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant.

Techniques Used: Injection, Saline, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Western Blot, Activity Assay

GDF7 inhibits inflammation and oxidative stress in LPS-treated mice. (a) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI. After 12 h, BALF was collected for the analysis of total cells, neutrophils, and macrophages ( n = 6). (b) MPO activity in lung tissues ( n = 6). (c) IL-6 and TNF- α levels in lung tissues ( n = 6). (d) ROS content in lung tissues ( n = 6). (e) MDA and 4-HNE levels in lung tissues ( n = 6). (f-g) Total SOD activity and GSH content in lung tissues ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant.

Figure Legend Snippet: GDF7 inhibits inflammation and oxidative stress in LPS-treated mice. (a) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI. After 12 h, BALF was collected for the analysis of total cells, neutrophils, and macrophages ( n = 6). (b) MPO activity in lung tissues ( n = 6). (c) IL-6 and TNF- α levels in lung tissues ( n = 6). (d) ROS content in lung tissues ( n = 6). (e) MDA and 4-HNE levels in lung tissues ( n = 6). (f-g) Total SOD activity and GSH content in lung tissues ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant.

Techniques Used: Injection, Saline, Activity Assay

GDF7 reduces LPS-stimulated inflammation and oxidative stress in primary macrophages. (a) Primary peritoneal macrophages were pretreated with rmGDF7 (10 nmol/L) for 24 h and then stimulated with LPS (100 ng/mL) for another 6 h. Cell medium was collected for the analysis of IL-6 and TNF- α ( n = 6). (b) ROS content in macrophages ( n = 6). (c) MDA and 4-HNE levels in macrophages ( n = 6). (d-e) Total SOD activity and GSH content in macrophages ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant.

Figure Legend Snippet: GDF7 reduces LPS-stimulated inflammation and oxidative stress in primary macrophages. (a) Primary peritoneal macrophages were pretreated with rmGDF7 (10 nmol/L) for 24 h and then stimulated with LPS (100 ng/mL) for another 6 h. Cell medium was collected for the analysis of IL-6 and TNF- α ( n = 6). (b) ROS content in macrophages ( n = 6). (c) MDA and 4-HNE levels in macrophages ( n = 6). (d-e) Total SOD activity and GSH content in macrophages ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant.

Techniques Used: Activity Assay

GDF7 attenuates LPS-induced ALI through activating AMPK in vivo. (a) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI. After 12 h, lung tissues were harvested for the analysis of the AMPK pathway using western blot ( n = 6). (b) To inhibit AMPK, ALI mice were intraperitoneally injected with CpC (20 mg/kg) at 2 h pre- and 2 h post-rmGDF7 injection, and then IL-6 and TNF- α levels in lung tissues were detected ( n = 6). (c) ROS content in lung tissues ( n = 6). (d) MDA and 4-HNE levels in lung tissues ( n = 6). (e) LDH activity in lung tissues ( n = 6). (f) Lung wet to dry ratio ( n = 6). (g) Arterial blood gas analysis results ( n = 6). (h) Respiratory function was detected by Buxco, including pulmonary ventilation, lung compliance, and airway resistance ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant.

Figure Legend Snippet: GDF7 attenuates LPS-induced ALI through activating AMPK in vivo. (a) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI. After 12 h, lung tissues were harvested for the analysis of the AMPK pathway using western blot ( n = 6). (b) To inhibit AMPK, ALI mice were intraperitoneally injected with CpC (20 mg/kg) at 2 h pre- and 2 h post-rmGDF7 injection, and then IL-6 and TNF- α levels in lung tissues were detected ( n = 6). (c) ROS content in lung tissues ( n = 6). (d) MDA and 4-HNE levels in lung tissues ( n = 6). (e) LDH activity in lung tissues ( n = 6). (f) Lung wet to dry ratio ( n = 6). (g) Arterial blood gas analysis results ( n = 6). (h) Respiratory function was detected by Buxco, including pulmonary ventilation, lung compliance, and airway resistance ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant.

Techniques Used: In Vivo, Injection, Saline, Western Blot, Activity Assay

GDF7 attenuates LPS-induced ALI through activating AMPK in vitro. (a) Primary peritoneal macrophages were pretreated with rmGDF7 (10 nmol/L) for 24 h and then stimulated with LPS (100 ng/mL) for another 6 h. To inhibit AMPK, macrophages were pretreated with CpC (10 μ mol/L) for 12 h before LPS stimulation. Cell medium was collected for the analysis of IL-6 and TNF- α ( n = 6). (b) ROS content in macrophages ( n = 6). (c) MDA and 4-HNE levels in macrophages ( n = 6). (d-e) Total SOD activity and GSH content in macrophages ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant.

Figure Legend Snippet: GDF7 attenuates LPS-induced ALI through activating AMPK in vitro. (a) Primary peritoneal macrophages were pretreated with rmGDF7 (10 nmol/L) for 24 h and then stimulated with LPS (100 ng/mL) for another 6 h. To inhibit AMPK, macrophages were pretreated with CpC (10 μ mol/L) for 12 h before LPS stimulation. Cell medium was collected for the analysis of IL-6 and TNF- α ( n = 6). (b) ROS content in macrophages ( n = 6). (c) MDA and 4-HNE levels in macrophages ( n = 6). (d-e) Total SOD activity and GSH content in macrophages ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant.

Techniques Used: In Vitro, Activity Assay

GDF7 activates AMPK through downregulating STING in vivo. (a) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI. After 12 h, lung tissues were harvested for the analysis of STING using western blot ( n = 6). (b) To investigate the involvement of STING, STING KO mice were used, and lung tissues were harvested for the analysis of the AMPK pathway using western blot ( n = 6). (c) IL-6 and TNF- α levels in the lung tissues ( n = 6). (d) ROS content in lung tissues ( n = 6). (e) LDH activity in lung tissues ( n = 6). (f) Lung wet to dry ratio ( n = 6). (g) Arterial blood gas analysis results ( n = 6). (h) Respiratory function was detected by Buxco, including pulmonary ventilation, lung compliance, and airway resistance ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant. NS indicated no statistical significance.

Figure Legend Snippet: GDF7 activates AMPK through downregulating STING in vivo. (a) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI. After 12 h, lung tissues were harvested for the analysis of STING using western blot ( n = 6). (b) To investigate the involvement of STING, STING KO mice were used, and lung tissues were harvested for the analysis of the AMPK pathway using western blot ( n = 6). (c) IL-6 and TNF- α levels in the lung tissues ( n = 6). (d) ROS content in lung tissues ( n = 6). (e) LDH activity in lung tissues ( n = 6). (f) Lung wet to dry ratio ( n = 6). (g) Arterial blood gas analysis results ( n = 6). (h) Respiratory function was detected by Buxco, including pulmonary ventilation, lung compliance, and airway resistance ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant. NS indicated no statistical significance.

Techniques Used: In Vivo, Injection, Saline, Western Blot, Activity Assay

GDF7 activates AMPK through downregulating STING in vitro. (a) Primary peritoneal macrophages were pretreated with rmGDF7 (10 nmol/L) for 24 h and then stimulated with LPS (100 ng/mL) for another 6 h. To investigate the involvement of STING, STING KO macrophages were used. Cells were harvested for the analysis of the AMPK pathway using western blot ( n = 6). (b) Cell medium was collected for the analysis of IL-6 and TNF- α ( n = 6). (c) ROS content in macrophages ( n = 6). (d) MDA and 4-HNE levels in macrophages ( n = 6). (e-f) Total SOD activity and GSH content in macrophages ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant. NS indicated no statistical significance.

Figure Legend Snippet: GDF7 activates AMPK through downregulating STING in vitro. (a) Primary peritoneal macrophages were pretreated with rmGDF7 (10 nmol/L) for 24 h and then stimulated with LPS (100 ng/mL) for another 6 h. To investigate the involvement of STING, STING KO macrophages were used. Cells were harvested for the analysis of the AMPK pathway using western blot ( n = 6). (b) Cell medium was collected for the analysis of IL-6 and TNF- α ( n = 6). (c) ROS content in macrophages ( n = 6). (d) MDA and 4-HNE levels in macrophages ( n = 6). (e-f) Total SOD activity and GSH content in macrophages ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant. NS indicated no statistical significance.

Techniques Used: In Vitro, Western Blot, Activity Assay

Figure Legend Snippet: Schematic diagram of the molecular mechanisms underlying GDF7-regulatedsepsis-induced ALI.

Techniques Used:

Recombinant:

Article Title: Growth Differentiation Factor 7 Prevents Sepsis-Induced Acute Lung Injury in Mice
Article Snippet: LPS ( Escherichia coli O111: B4, #L2630) was purchased from Sigma-Aldrich (St Louis, MO, USA). .. Recombinant Mouse GDF7 Protein (rmGDF7, #779-G7), Mouse IL-6 Quantikine ELISA Kit (#M6000B), and Mouse TNF- α Quantikine ELISA Kit (#MTA00B) were purchased from R&D Systems, Inc. (Minneapolis, MN, Canada). .. Compound C (CpC, #HY-13418A) was purchased from MedChemExpress (Monmouth Junction, NJ, USA), and the structure was provided on the official website: https://www.medchemexpress.cn/dorsomorphin.html .

Enzyme-linked Immunosorbent Assay:

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Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Growth Differentiation Factor 7 Prevents Sepsis-Induced Acute Lung Injury in Mice

doi: 10.1155/2022/3676444

Figure Lengend Snippet: GDF7 alleviates LPS-induced ALI and pulmonary dysfunction in mice. (a) Mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI, and serum GDF7 levels were detected using an ELISA kit after 12 h ( n = 6). (b) The levels of Gdf7 mRNA in lung tissues with or without LPS instillation were detected using quantitative real-time PCR ( n = 6). (c) The levels of GDF7 protein in lung tissues with or without LPS instillation were detected using western blot ( n = 6). (d-e) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and serum and lung GDF7 levels in mice were detected using an ELISA kit after 24 h ( n = 6). (f) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI. After 12 h, fresh lungs were harvested for the analysis of LDH activity using a commercial kit ( n = 6). (g) Lung wet to dry ratio ( n = 6). (h) Total proteins in BALF ( n = 6). (i) Arterial blood gas analysis results ( n = 6). (j) Respiratory function was detected by Buxco, including pulmonary ventilation, lung compliance, and airway resistance ( n = 6). (k) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with a lethal dose of LPS (25 mg/kg). The survival rate was monitored every 12 h post-LPS treatment ( n = 20). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant.

Article Snippet: Recombinant Mouse GDF7 Protein (rmGDF7, #779-G7), Mouse IL-6 Quantikine ELISA Kit (#M6000B), and Mouse TNF- α Quantikine ELISA Kit (#MTA00B) were purchased from R&D Systems, Inc. (Minneapolis, MN, Canada).

Techniques: Injection, Saline, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Western Blot, Activity Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Growth Differentiation Factor 7 Prevents Sepsis-Induced Acute Lung Injury in Mice

doi: 10.1155/2022/3676444

Figure Lengend Snippet: GDF7 inhibits inflammation and oxidative stress in LPS-treated mice. (a) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI. After 12 h, BALF was collected for the analysis of total cells, neutrophils, and macrophages ( n = 6). (b) MPO activity in lung tissues ( n = 6). (c) IL-6 and TNF- α levels in lung tissues ( n = 6). (d) ROS content in lung tissues ( n = 6). (e) MDA and 4-HNE levels in lung tissues ( n = 6). (f-g) Total SOD activity and GSH content in lung tissues ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant.

Techniques: Injection, Saline, Activity Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Growth Differentiation Factor 7 Prevents Sepsis-Induced Acute Lung Injury in Mice

doi: 10.1155/2022/3676444

Figure Lengend Snippet: GDF7 reduces LPS-stimulated inflammation and oxidative stress in primary macrophages. (a) Primary peritoneal macrophages were pretreated with rmGDF7 (10 nmol/L) for 24 h and then stimulated with LPS (100 ng/mL) for another 6 h. Cell medium was collected for the analysis of IL-6 and TNF- α ( n = 6). (b) ROS content in macrophages ( n = 6). (c) MDA and 4-HNE levels in macrophages ( n = 6). (d-e) Total SOD activity and GSH content in macrophages ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant.

Techniques: Activity Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Growth Differentiation Factor 7 Prevents Sepsis-Induced Acute Lung Injury in Mice

doi: 10.1155/2022/3676444

Figure Lengend Snippet: GDF7 attenuates LPS-induced ALI through activating AMPK in vivo. (a) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI. After 12 h, lung tissues were harvested for the analysis of the AMPK pathway using western blot ( n = 6). (b) To inhibit AMPK, ALI mice were intraperitoneally injected with CpC (20 mg/kg) at 2 h pre- and 2 h post-rmGDF7 injection, and then IL-6 and TNF- α levels in lung tissues were detected ( n = 6). (c) ROS content in lung tissues ( n = 6). (d) MDA and 4-HNE levels in lung tissues ( n = 6). (e) LDH activity in lung tissues ( n = 6). (f) Lung wet to dry ratio ( n = 6). (g) Arterial blood gas analysis results ( n = 6). (h) Respiratory function was detected by Buxco, including pulmonary ventilation, lung compliance, and airway resistance ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant.

Techniques: In Vivo, Injection, Saline, Western Blot, Activity Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Growth Differentiation Factor 7 Prevents Sepsis-Induced Acute Lung Injury in Mice

doi: 10.1155/2022/3676444

Figure Lengend Snippet: GDF7 attenuates LPS-induced ALI through activating AMPK in vitro. (a) Primary peritoneal macrophages were pretreated with rmGDF7 (10 nmol/L) for 24 h and then stimulated with LPS (100 ng/mL) for another 6 h. To inhibit AMPK, macrophages were pretreated with CpC (10 μ mol/L) for 12 h before LPS stimulation. Cell medium was collected for the analysis of IL-6 and TNF- α ( n = 6). (b) ROS content in macrophages ( n = 6). (c) MDA and 4-HNE levels in macrophages ( n = 6). (d-e) Total SOD activity and GSH content in macrophages ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant.

Techniques: In Vitro, Activity Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Growth Differentiation Factor 7 Prevents Sepsis-Induced Acute Lung Injury in Mice

doi: 10.1155/2022/3676444

Figure Lengend Snippet: GDF7 activates AMPK through downregulating STING in vivo. (a) Mice were subcutaneously injected with rmGDF7 (25 μ g per mouse), and 24 h later, ALI mice were intratracheally injected with LPS (5 mg/kg in 50 μ L saline) to generate sepsis-induced ALI. After 12 h, lung tissues were harvested for the analysis of STING using western blot ( n = 6). (b) To investigate the involvement of STING, STING KO mice were used, and lung tissues were harvested for the analysis of the AMPK pathway using western blot ( n = 6). (c) IL-6 and TNF- α levels in the lung tissues ( n = 6). (d) ROS content in lung tissues ( n = 6). (e) LDH activity in lung tissues ( n = 6). (f) Lung wet to dry ratio ( n = 6). (g) Arterial blood gas analysis results ( n = 6). (h) Respiratory function was detected by Buxco, including pulmonary ventilation, lung compliance, and airway resistance ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant. NS indicated no statistical significance.

Techniques: In Vivo, Injection, Saline, Western Blot, Activity Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Growth Differentiation Factor 7 Prevents Sepsis-Induced Acute Lung Injury in Mice

doi: 10.1155/2022/3676444

Figure Lengend Snippet: GDF7 activates AMPK through downregulating STING in vitro. (a) Primary peritoneal macrophages were pretreated with rmGDF7 (10 nmol/L) for 24 h and then stimulated with LPS (100 ng/mL) for another 6 h. To investigate the involvement of STING, STING KO macrophages were used. Cells were harvested for the analysis of the AMPK pathway using western blot ( n = 6). (b) Cell medium was collected for the analysis of IL-6 and TNF- α ( n = 6). (c) ROS content in macrophages ( n = 6). (d) MDA and 4-HNE levels in macrophages ( n = 6). (e-f) Total SOD activity and GSH content in macrophages ( n = 6). All data were presented as mean ± SD, and ∗ P < 0.05 was regarded to be statistically significant. NS indicated no statistical significance.

Techniques: In Vitro, Western Blot, Activity Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Growth Differentiation Factor 7 Prevents Sepsis-Induced Acute Lung Injury in Mice

doi: 10.1155/2022/3676444

Figure Lengend Snippet: Schematic diagram of the molecular mechanisms underlying GDF7-regulatedsepsis-induced ALI.

Techniques:

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recombinant mouse gdf7 protein (R&D Systems)

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